Researchers commonly employ fluorescence microscopy to characterize molecular, cellular, and developmental processes in depth. Despite its benefits, scientists can face challenges when imaging their samples, such as bleed-through, photobleaching, high background signal, phototoxicity, and channel misalignment.
Download this poster from The Scientist’s Creative Services Team and Evident to view simulated microscopy images demonstrating the five major problems that arise during fluorescence imaging and to explore strategies to overcome these obstacles.